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Cattle IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
ELK7953
Size： Price：
48 T $412.00
96 T $588.00
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Overview

Product name: Cattle IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
Reactivity: Cattle
Alternative Names: IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
Assay Type: Sandwich
Sensitivity: 6.3 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.035 1.949
500.00 1.700 1.614
250.00 1.185 1.099
125.00 0.857 0.771
62.50 0.655 0.569
31.25 0.256 0.170
15.63 0.291 0.205
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-2sRa/CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2sRa/CD25 in samples.
Matrix Recovery range Average
serum(n=5) 80-93% 86%
EDTA plasma(n=5) 78-90% 84%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2sRa/CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 82-96% 87-103% 95-102%
EDTA plasma(n=5) 86-97% 90-98% 80-93% 92-105%
Heparin plasma(n=5) 87-98% 85-92% 79-96% 89-100%