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Human Anti-GAD(Anti-Glutamic Acid Decarboxylase Antibody) ELISA Kit
ELK7911
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-GAD(Anti-Glutamic Acid Decarboxylase Antibody) ELISA Kit
Reactivity: Human
Alternative Names: Glutamate Decarboxylase
Assay Type: Competitive Inhibition
Sensitivity: 0.59 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2.5h
Research Area: Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-GAD protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GAD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GAD in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 0.227
50.00 0.386
25.00 0.596
12.50 0.823
6.25 1.112
3.13 1.592
1.57 1.796
0.00 2.213

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-GAD and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-GAD in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-GAD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-106% 85-101% 87-102% 89-95%
EDTA plasma(n=5) 88-97% 86-92% 91-99% 79-97%
Heparin plasma(n=5) 85-90% 83-96% 95-102% 88-95%