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IAA(Indole 3 Acetic Acid) ELISA Kit
ELK7852
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48 T $265.00
96 T $376.00
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Overview

Product name: IAA(Indole 3 Acetic Acid) ELISA Kit
Reactivity: General
Alternative Names: IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin
Assay Type: Competitive Inhibition
Sensitivity: 0.89 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: tissue or cell culture supernates
Assay Length: 2h
Research Area: Signal transduction;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IAA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IAA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.211
100.00 0.389
50.00 0.621
25.00 0.861
12.50 1.152
6.25 1.565
3.13 1.789
0.00 2.123

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IAA and the recovery rates were calculated by comparing the measured value to the expected amount of IAA in samples.
Matrix Recovery range Average
serum(n=5) 83-99% 95%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 85-97% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 91-99% 83-97% 98-105% 96-101%
EDTA plasma(n=5) 85-92% 79-96% 89-101% 85-97%
Heparin plasma(n=5) 92-105% 86-98% 80-93% 89-97%