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1,5-AG(1,5-Anhydroglucitol) ELISA Kit
ELK7840
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48 T $265.00
96 T $376.00
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Overview

Product name: 1,5-AG(1,5-Anhydroglucitol) ELISA Kit
Reactivity: General
Alternative Names: LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol
Assay Type: Competitive Inhibition
Sensitivity: 0.6 μg/mL
Standard: 150 μg/mL
Detection Range: 2.35-150 μg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (μg/mL) OD Corrected OD
150.00 0.175
75.00 0.356
37.50 0.672
18.75 0.913
9.38 1.253
4.69 1.587
2.35 1.799
0.00 2.101

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 1,5-AG and the recovery rates were calculated by comparing the measured value to the expected amount of 1,5-AG in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 84-102% 93%
Heparin plasma(n=5) 87-95% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 1,5-AG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 87-96% 86-97% 88-95%
EDTA plasma(n=5) 88-102% 87-102% 85-96% 87-101%
Heparin plasma(n=5) 87-96% 81-93% 86-98% 91-103%