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Human Ang1-7(Angiotensin 1-7) ELISA Kit
ELK7678
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Ang1-7(Angiotensin 1-7) ELISA Kit
Reactivity: Human
Alternative Names: Ang1-7
Assay Type: Sandwich
Sensitivity: 4.69 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ang1-7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ang1-7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ang1-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ang1-7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 1.946 1.864
500.00 1.587 1.505
250.00 1.157 1.075
125.00 0.897 0.815
62.50 0.508 0.426
31.25 0.385 0.303
15.63 0.226 0.144
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Ang1-7 and the recovery rates were calculated by comparing the measured value to the expected amount of Ang1-7 in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 88-102% 95%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ang1-7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 91-99% 83-89% 88-94% 87-93%
EDTA plasma(n=5) 89-101% 85-96% 95-104% 82-90%
Heparin plasma(n=5) 95-104% 97-106% 85-98% 81-92%