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Rat Ang1-7(Angiotensin 1-7) ELISA Kit
ELK7677
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat Ang1-7(Angiotensin 1-7) ELISA Kit
Reactivity: Rat
Alternative Names: Ang1-7
Assay Type: Sandwich
Sensitivity: 0.91 pg/mL
Standard: 200 pg/mL
Detection Range: 3.13-200 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Ang1-7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Ang1-7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Ang1-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Ang1-7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
200.00 1.972 1.886
100.00 1.554 1.468
50.00 1.185 1.099
25.00 0.831 0.745
12.50 0.567 0.481
6.25 0.386 0.300
3.13 0.189 0.103
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Ang1-7 and the recovery rates were calculated by comparing the measured value to the expected amount of Ang1-7 in samples.
Matrix Recovery range Average
serum(n=5) 87-97% 90%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 88-102% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ang1-7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 85-97% 85-94% 87-96%
EDTA plasma(n=5) 89-104% 97-106% 82-91% 85-96%
Heparin plasma(n=5) 85-93% 87-96% 97-105% 95-102%