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Human MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit
ELK7627
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48 T $320.00
96 T $458.00
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Overview

Product name: Human MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit
Reactivity: Human
Alternative Names: MOTS-c
Assay Type: Competitive Inhibition
Sensitivity: 0.99 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Developmental science;
Uniprot ID: A0A0C5B5G6
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MOTS-c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.179
100.00 0.336
50.00 0.655
25.00 0.895
12.50 1.177
6.25 1.572
3.13 1.813
0.00 2.325

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MOTS-c and the recovery rates were calculated by comparing the measured value to the expected amount of MOTS-c in samples.
Matrix Recovery range Average
serum(n=5) 85-98% 91%
EDTA plasma(n=5) 96-107% 102%
Heparin plasma(n=5) 80-95% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MOTS-c and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-96% 85-97% 89-100% 85-96%
EDTA plasma(n=5) 85-94% 87-101% 86-97% 88-95%
Heparin plasma(n=5) 98-106% 86-102% 79-93% 89-97%