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Cattle VIP(Vasoactive Intestinal Peptide) ELISA Kit
ELK7544
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48 T $412.00
96 T $588.00
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Overview

Product name: Cattle VIP(Vasoactive Intestinal Peptide) ELISA Kit
Reactivity: Cattle
Alternative Names: PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
Assay Type: Sandwich
Sensitivity: 2.63 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Gastroenterology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle VIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle VIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle VIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle VIP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.166 2.072
250.00 1.507 1.413
125.00 1.185 1.091
62.50 0.857 0.763
31.25 0.473 0.379
15.63 0.313 0.219
7.82 0.212 0.118
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant VIP and the recovery rates were calculated by comparing the measured value to the expected amount of VIP in samples.
Matrix Recovery range Average
serum(n=5) 80-96% 88%
EDTA plasma(n=5) 80-92% 86%
Heparin plasma(n=5) 78-95% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 91-102% 81-97% 93-107%
EDTA plasma(n=5) 88-102% 87-102% 85-96% 87-101%
Heparin plasma(n=5) 87-94% 85-96% 89-100% 85-96%