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Rat TCN2(Transcobalamin II, Macrocytic Anemia) ELISA Kit
ELK7374
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat TCN2(Transcobalamin II, Macrocytic Anemia) ELISA Kit
Reactivity: Rat
Alternative Names: TC2; Transcobalamin II
Assay Type: Sandwich
Sensitivity: 29 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Metabolic pathway;
Uniprot ID: Q9R0D6
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TCN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TCN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TCN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TCN2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.379 2.288
2500.00 1.636 1.545
1250.00 1.223 1.132
625.00 0.815 0.724
312.50 0.611 0.520
156.25 0.352 0.261
78.13 0.208 0.117
0.00 0.091 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TCN2 and the recovery rates were calculated by comparing the measured value to the expected amount of TCN2 in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TCN2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-96% 87-101% 88-102% 87-102%
EDTA plasma(n=5) 93-102% 81-96% 93-106% 82-95%
Heparin plasma(n=5) 87-98% 92-104% 82-96% 95-102%