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Horse D2D(D-Dimer) ELISA Kit
ELK7180
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48 T $412.00
96 T $588.00
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Overview

Product name: Horse D2D(D-Dimer) ELISA Kit
Reactivity: Horse
Alternative Names: D 2 Dimer
Assay Type: Sandwich
Sensitivity: 27.2 ng/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 ng/mL
Sample Type: Plasma
Assay Length: 2h
Research Area: Hematology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse D2D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse D2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse D2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse D2D in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
5000.00 1.948 1.855
2500.00 1.642 1.549
1250.00 1.139 1.046
625.00 0.797 0.704
312.50 0.527 0.434
156.25 0.319 0.226
78.13 0.227 0.134
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant D2D and the recovery rates were calculated by comparing the measured value to the expected amount of D2D in samples.
Matrix Recovery range Average
serum(n=5) 86-98% 92%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 82-95% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of D2D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 82-96% 86-97% 93-101%
EDTA plasma(n=5) 87-96% 86-95% 91-103% 87-93%
Heparin plasma(n=5) 95-103% 93-105% 91-99% 83-96%