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Rat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
ELK7048
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
Reactivity: Rat
Alternative Names: CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5
Assay Type: Sandwich
Sensitivity: 27 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VE-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VE-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VE-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 1.946 1.850
2500.00 1.527 1.431
1250.00 1.088 0.992
625.00 0.682 0.586
312.50 0.539 0.443
156.25 0.305 0.209
78.13 0.249 0.153
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant VE-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of VE-Cadherin in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 94-107% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VE-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-95% 85-98% 82-97% 92-101%
EDTA plasma(n=5) 84-94% 85-103% 94-105% 92-101%
Heparin plasma(n=5) 81-94% 96-105% 92-103% 82-96%