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Rat SALb(Salusin Beta) ELISA Kit
ELK7018
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat SALb(Salusin Beta) ELISA Kit
Reactivity: Rat
Alternative Names: Salusin Beta
Assay Type: Competitive Inhibition
Sensitivity: 1.94 pg/mL
Standard: 400 pg/mL
Detection Range: 6.25-400 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SALb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SALb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SALb in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
400.00 0.227
200.00 0.386
100.00 0.611
50.00 0.833
25.00 1.152
12.50 1.511
6.25 1.775
0.00 2.123

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant SALb and the recovery rates were calculated by comparing the measured value to the expected amount of SALb in samples.
Matrix Recovery range Average
serum(n=5) 88-96% 92%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 81-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SALb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 78-92% 87-98% 85-97%
EDTA plasma(n=5) 78-93% 96-102% 95-103% 89-97%
Heparin plasma(n=5) 88-97% 86-92% 91-103% 89-97%