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Mouse ADORA2b(Adenosine A2b Receptor) ELISA Kit
ELK6990
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse ADORA2b(Adenosine A2b Receptor) ELISA Kit
Reactivity: Mouse
Alternative Names: ADORA2B; Adenosine receptor A2b
Assay Type: Sandwich
Sensitivity: 30 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;
Uniprot ID: Q60614
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ADORA2b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ADORA2b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ADORA2b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ADORA2b in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.172 2.084
2500.00 1.628 1.540
1250.00 1.283 1.195
625.00 0.877 0.789
312.50 0.547 0.459
156.25 0.389 0.301
78.13 0.168 0.080
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ADORA2b and the recovery rates were calculated by comparing the measured value to the expected amount of ADORA2b in samples.
Matrix Recovery range Average
serum(n=5) 90-103% 96%
EDTA plasma(n=5) 78-93% 85%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADORA2b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 86-92% 91-99% 79-97%
EDTA plasma(n=5) 87-96% 85-97% 85-94% 87-96%
Heparin plasma(n=5) 85-97% 87-96% 85-96% 85-95%