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Rat INSL3(Insulin Like Protein 3) ELISA Kit
ELK6400
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat INSL3(Insulin Like Protein 3) ELISA Kit
Reactivity: Rat
Alternative Names: RLNL; RLF; Relaxin-Like Factor,Leydig Cell; Leydig insulin-like peptide
Assay Type: Competitive Inhibition
Sensitivity: 62.8 pg/mL
Standard: 15000 pg/mL
Detection Range: 234.38-15000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Endocrinology;
Uniprot ID: Q9WUK0
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat INSL3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat INSL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat INSL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
15000.00 0.185
7500.00 0.389
3750.00 0.621
1875.00 0.833
937.50 1.197
468.75 1.522
234.38 1.755
0.00 2.325

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant INSL3 and the recovery rates were calculated by comparing the measured value to the expected amount of INSL3 in samples.
Matrix Recovery range Average
serum(n=5) 88-99% 93%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 83-97% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of INSL3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-105% 90-101% 87-98% 93-101%
EDTA plasma(n=5) 88-102% 86-92% 91-103% 89-97%
Heparin plasma(n=5) 86-93% 86-97% 85-103% 89-97%