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Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit
ELK5860
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48 T $412.00
96 T $588.00
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Overview

Product name: Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit
Reactivity: Cattle
Alternative Names: BP4; HT29-IGFBP; IBP4
Assay Type: Competitive Inhibition
Sensitivity: 29.62 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: Serum, plasma and other biological fluids..
Assay Length: 2h
Research Area: Apoptosis;Tumor immunity;
Uniprot ID: Q05716
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle IGFBP4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
6000.00 0.197
3000.00 0.385
1500.00 0.567
750.00 0.878
375.00 1.157
187.50 1.592
93.75 1.715
0.00 2.112

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IGFBP4 and the recovery rates were calculated by comparing the measured value to the expected amount of IGFBP4 in samples.
Matrix Recovery range Average
serum(n=5) 78-93% 85%
EDTA plasma(n=5) 78-95% 86%
Heparin plasma(n=5) 85-97% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGFBP4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 79-96% 87-98% 95-102%
EDTA plasma(n=5) 98-105% 96-103% 87-95% 83-92%
Heparin plasma(n=5) 87-98% 89-103% 82-95% 86-99%