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Rat sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
ELK5806
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
Reactivity: Rat
Alternative Names: MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9
Assay Type: Sandwich
Sensitivity: 13.7 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 1.966 1.882
1000.00 1.628 1.544
500.00 1.052 0.968
250.00 0.819 0.735
125.00 0.457 0.373
62.50 0.324 0.240
31.25 0.258 0.174
0.00 0.084 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sC5b-9 and the recovery rates were calculated by comparing the measured value to the expected amount of sC5b-9 in samples.
Matrix Recovery range Average
serum(n=5) 80-92% 86%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 88-102% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sC5b-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 86-92% 89-97% 91-103%
EDTA plasma(n=5) 86-94% 91-105% 87-99% 95-100%
Heparin plasma(n=5) 82-95% 83-96% 88-95% 96-102%