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Human 20S-PSM(20S-Proteasome) ELISA Kit
ELK5359
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48 T $320.00
96 T $458.00
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Overview

Product name: Human 20S-PSM(20S-Proteasome) ELISA Kit
Reactivity: Human
Alternative Names: CP; 20SPSM; 20S Core Particle
Assay Type: Sandwich
Sensitivity: 0.66 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 20S-PSM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 20S-PSM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.168 2.077
50.00 1.615 1.524
25.00 1.286 1.195
12.50 0.806 0.715
6.25 0.421 0.330
3.13 0.354 0.263
1.57 0.262 0.171
0.00 0.091 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 20S-PSM and the recovery rates were calculated by comparing the measured value to the expected amount of 20S-PSM in samples.
Matrix Recovery range Average
serum(n=5) 80-96% 88%
EDTA plasma(n=5) 82-98% 90%
Heparin plasma(n=5) 84-98% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 20S-PSM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 93-106% 87-101% 91-104%
EDTA plasma(n=5) 85-94% 87-101% 89-99% 85-95%
Heparin plasma(n=5) 86-94% 93-102% 89-97% 85-94%