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Human ECE2(Endothelin Converting Enzyme 2) ELISA Kit
ELK5083
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48 T $320.00
96 T $458.00
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Overview

Product name: Human ECE2(Endothelin Converting Enzyme 2) ELISA Kit
Reactivity: Human
Alternative Names: Methyltransferase-like region
Assay Type: Competitive Inhibition
Sensitivity: 45.9 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 2h
Research Area: Enzyme & Kinase;
Uniprot ID: P0DPD6
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ECE2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ECE2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
10000.00 0.211
5000.00 0.389
2500.00 0.655
1250.00 0.895
625.00 1.119
312.50 1.522
156.25 1.762
0.00 2.101

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ECE2 and the recovery rates were calculated by comparing the measured value to the expected amount of ECE2 in samples.
Matrix Recovery range Average
serum(n=5) 88-102% 95%
EDTA plasma(n=5) 87-97% 90%
Heparin plasma(n=5) 90-105% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ECE2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-94% 96-105% 93-106% 82-95%
EDTA plasma(n=5) 86-97% 83-96% 91-103% 96-105%
Heparin plasma(n=5) 85-100% 98-106% 86-99% 78-95%