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Human KLK1(Kallikrein 1) ELISA Kit
ELK4888
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48 T $320.00
96 T $458.00
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Overview

Product name: Human KLK1(Kallikrein 1) ELISA Kit
Reactivity: Human
Alternative Names: KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein
Assay Type: Competitive Inhibition
Sensitivity: 27.51 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Enzyme & Kinase;Tumor immunity;Hepatology;
Uniprot ID: P06870
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
6000.00 0.197
3000.00 0.397
1500.00 0.551
750.00 0.897
375.00 1.297
187.50 1.589
93.75 1.863
0.00 2.219

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant KLK1 and the recovery rates were calculated by comparing the measured value to the expected amount of KLK1 in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 87-93% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KLK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-96% 85-97% 89-100% 85-96%
EDTA plasma(n=5) 89-98% 83-103% 98-105% 92-101%
Heparin plasma(n=5) 97-105% 80-96% 93-105% 90-99%