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Human IGFIIE(Pro-Insulin Like Growth Factor II E-Peptide) ELISA Kit
ELK4859
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48 T $320.00
96 T $458.00
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Overview

Product name: Human IGFIIE(Pro-Insulin Like Growth Factor II E-Peptide) ELISA Kit
Reactivity: Human
Alternative Names: Preptin; Proinsulin-Like Growth Factor-II
Assay Type: Competitive Inhibition
Sensitivity: 13.5 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 2h
Research Area: Infection immunity;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human IGFIIE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGFIIE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFIIE in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
3000.00 0.221
1500.00 0.411
750.00 0.715
375.00 0.933
187.50 1.225
93.75 1.587
46.88 1.761
0.00 2.211

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IGFIIE and the recovery rates were calculated by comparing the measured value to the expected amount of IGFIIE in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 78-91% 84%
Heparin plasma(n=5) 88-101% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGFIIE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-94% 85-96% 97-101% 89-102%
EDTA plasma(n=5) 86-93% 91-103% 87-98% 92-99%
Heparin plasma(n=5) 85-92% 79-86% 87-103% 85-97%