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Rat CD163(Cluster Of Differentiation) ELISA Kit
ELK4826
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat CD163(Cluster Of Differentiation) ELISA Kit
Reactivity: Rat
Alternative Names: M130; MM130; Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor
Assay Type: Sandwich
Sensitivity: 6.9 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD163. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD163. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD163, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD163 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.119 2.016
500.00 1.527 1.424
250.00 1.174 1.071
125.00 0.872 0.769
62.50 0.543 0.440
31.25 0.395 0.292
15.63 0.205 0.102
0.00 0.103 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CD163 and the recovery rates were calculated by comparing the measured value to the expected amount of CD163 in samples.
Matrix Recovery range Average
serum(n=5) 77-95% 86%
EDTA plasma(n=5) 92-106% 99%
Heparin plasma(n=5) 88-103% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD163 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 93-102% 79-94% 87-98%
EDTA plasma(n=5) 86-93% 91-105% 87-98% 89-94%
Heparin plasma(n=5) 81-94% 83-96% 87-98% 85-97%