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Mouse FDP(Fibrinogen Degradation Product) ELISA Kit
ELK4809
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse FDP(Fibrinogen Degradation Product) ELISA Kit
Reactivity: Mouse
Alternative Names: FnDP; Fibrin Split Products; Fibrin Degradation Product
Assay Type: Sandwich
Sensitivity: 24.9 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: plasma
Assay Length: 3.5h
Research Area: Metabolic pathway;Hematology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FDP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FDP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FDP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FDP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 2.123 2.035
2000.00 1.579 1.491
1000.00 1.168 1.080
500.00 0.797 0.709
250.00 0.548 0.460
125.00 0.361 0.273
62.50 0.236 0.148
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant FDP and the recovery rates were calculated by comparing the measured value to the expected amount of FDP in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 85-99% 92%
Heparin plasma(n=5) 93-107% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FDP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 85-96% 87-101% 89-97%
EDTA plasma(n=5) 85-101% 84-98% 85-93% 86-98%
Heparin plasma(n=5) 85-104% 79-92% 83-95% 86-97%