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Mouse aEP(Alpha-Endorphin) ELISA Kit
ELK4388
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Mouse aEP(Alpha-Endorphin) ELISA Kit
Reactivity: Mouse
Alternative Names: AEP
Assay Type: Competitive Inhibition
Sensitivity: 4.22 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Signal transduction;Endocrinology;Neuro science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse aEP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse aEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse aEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 0.227
500.00 0.396
250.00 0.621
125.00 0.833
62.50 1.119
31.25 1.565
15.63 1.775
0.00 2.123

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant aEP and the recovery rates were calculated by comparing the measured value to the expected amount of aEP in samples.
Matrix Recovery range Average
serum(n=5) 84-106% 100%
EDTA plasma(n=5) 87-93% 90%
Heparin plasma(n=5) 80-90% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of aEP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-101% 89-98% 85-94%
EDTA plasma(n=5) 86-97% 93-105% 82-93% 95-104%
Heparin plasma(n=5) 85-102% 96-103% 87-98% 95-102%