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Human KLK14(Kallikrein 14) ELISA Kit
ELK3390
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48 T $320.00
96 T $458.00
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Overview

Product name: Human KLK14(Kallikrein 14) ELISA Kit
Reactivity: Human
Alternative Names: KLK-L6; Kallikrein-like protein 6
Assay Type: Competitive Inhibition
Sensitivity: 43.2 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 2h
Research Area: Enzyme & Kinase;
Uniprot ID: Q9P0G3
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK14 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK14 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
10000.00 0.232
5000.00 0.387
2500.00 0.692
1250.00 0.933
625.00 1.225
312.50 1.596
156.25 1.813
0.00 2.211

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant KLK14 and the recovery rates were calculated by comparing the measured value to the expected amount of KLK14 in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 94-107% 100%
Heparin plasma(n=5) 94-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KLK14 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-96% 85-97% 87-96%
EDTA plasma(n=5) 87-90% 87-98% 85-96% 85-95%
Heparin plasma(n=5) 83-96% 92-103% 87-98% 93-101%