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Human KLK12(Kallikrein 12) ELISA Kit
ELK3389
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48 T $320.00
96 T $458.00
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Overview

Product name: Human KLK12(Kallikrein 12) ELISA Kit
Reactivity: Human
Alternative Names: KLK-L5; KLKL5; Kallikrein-Related Peptidase 12; Kallikrein-like protein 5
Assay Type: Competitive Inhibition
Sensitivity: 7.14 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 2h
Research Area: Enzyme & Kinase;
Uniprot ID: Q9UKR0
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 0.185
500.00 0.397
250.00 0.689
125.00 0.913
62.50 1.139
31.25 1.578
15.63 1.755
0.00 2.325

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant KLK12 and the recovery rates were calculated by comparing the measured value to the expected amount of KLK12 in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 80-93% 86%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KLK12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-98% 91-103% 85-96% 87-101%
EDTA plasma(n=5) 88-101% 85-93% 89-97% 85-94%
Heparin plasma(n=5) 83-97% 91-103% 87-98% 95-101%