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Human GIF(Gastric Intrinsic Factor) ELISA Kit
ELK3212
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48 T $320.00
96 T $458.00
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Overview

Product name: Human GIF(Gastric Intrinsic Factor) ELISA Kit
Reactivity: Human
Alternative Names: TCN3; IF; IFMH; INF; Transcobalamin III; Vitamin B Synthesis
Assay Type: Competitive Inhibition
Sensitivity: 0.95 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Endocrinology;
Uniprot ID: P27352
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GIF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GIF in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.191
100.00 0.352
50.00 0.621
25.00 0.833
12.50 1.152
6.25 1.511
3.13 1.789
0.00 2.115

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant GIF and the recovery rates were calculated by comparing the measured value to the expected amount of GIF in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 83-99% 95%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 87-100% 85-93% 79-96%
EDTA plasma(n=5) 88-98% 87-103% 89-97% 85-94%
Heparin plasma(n=5) 85-92% 96-104% 90-98% 80-92%