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Cattle RETN(Resistin) ELISA Kit
ELK2970
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48 T $412.00
96 T $588.00
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Overview

Product name: Cattle RETN(Resistin) ELISA Kit
Reactivity: Cattle
Alternative Names: ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3
Assay Type: Sandwich
Sensitivity: 31 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;
Uniprot ID: Q762I5
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle RETN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle RETN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle RETN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle RETN in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.075 2.010
2500.00 1.502 1.437
1250.00 0.939 0.874
625.00 0.747 0.682
312.50 0.546 0.481
156.25 0.312 0.247
78.13 0.217 0.152
0.00 0.065 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant RETN and the recovery rates were calculated by comparing the measured value to the expected amount of RETN in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 78-90% 84%
Heparin plasma(n=5) 85-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RETN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 79-93% 94-106% 83-97%
EDTA plasma(n=5) 80-101% 87-96% 96-105% 95-103%
Heparin plasma(n=5) 87-96% 91-101% 87-98% 89-103%