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Human Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
ELK2631
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
Reactivity: Human
Alternative Names: ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Neuro science;
Uniprot ID: Q6UWV6
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.096 2.014
10.00 1.602 1.520
5.00 1.067 0.985
2.50 0.872 0.790
1.25 0.572 0.490
0.63 0.302 0.220
0.32 0.165 0.083
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Alk-Smase and the recovery rates were calculated by comparing the measured value to the expected amount of Alk-Smase in samples.
Matrix Recovery range Average
serum(n=5) 84-106% 100%
EDTA plasma(n=5) 87-93% 90%
Heparin plasma(n=5) 80-90% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alk-Smase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 96-105% 86-97% 85-101%
EDTA plasma(n=5) 85-94% 87-96% 92-105% 89-102%
Heparin plasma(n=5) 85-103% 83-96% 95-102% 87-98%