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Mouse Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
ELK2630
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
Reactivity: Mouse
Alternative Names: ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase
Assay Type: Sandwich
Sensitivity: 0.051 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Neuro science;
Uniprot ID: Q3TIW9
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.201 2.113
5.00 1.593 1.505
2.50 1.144 1.056
1.25 0.975 0.887
0.63 0.539 0.451
0.32 0.273 0.185
0.16 0.226 0.138
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Alk-Smase and the recovery rates were calculated by comparing the measured value to the expected amount of Alk-Smase in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 82-97% 89%
Heparin plasma(n=5) 95-108% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alk-Smase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-97% 96-104% 85-93% 89-97%
EDTA plasma(n=5) 85-94% 87-103% 97-105% 79-90%
Heparin plasma(n=5) 85-102% 78-92% 87-98% 95-102%