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Mouse PAI2(Plasminogen Activator Inhibitor 2) ELISA Kit
ELK2582
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse PAI2(Plasminogen Activator Inhibitor 2) ELISA Kit
Reactivity: Mouse
Alternative Names: SERPINB2; PLANH2; Serpin Peptidase Inhibitor Clade B Member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor
Assay Type: Sandwich
Sensitivity: 3.2 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: plasma
Assay Length: 3.5h
Research Area: Hematology;Reproductive science;
Uniprot ID: P12388
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.121 2.032
250.00 1.643 1.554
125.00 1.183 1.094
62.50 0.755 0.666
31.25 0.593 0.504
15.63 0.300 0.211
7.82 0.196 0.107
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PAI2 and the recovery rates were calculated by comparing the measured value to the expected amount of PAI2 in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 80-93% 86%
Heparin plasma(n=5) 94-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PAI2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 83-96% 95-107% 87-102%
EDTA plasma(n=5) 95-104% 93-102% 79-94% 87-98%
Heparin plasma(n=5) 89-100% 79-91% 89-103% 82-95%