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Mouse EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit
ELK2479
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit
Reactivity: Mouse
Alternative Names: PK1; EGVEGF; PRK1, PROK1; Prokineticin 1; Black Mamba Toxin-Related Protein; Mambakine
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;
Uniprot ID: Q14A28
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 1.941 1.845
500.00 1.694 1.598
250.00 1.106 1.010
125.00 0.847 0.751
62.50 0.527 0.431
31.25 0.325 0.229
15.63 0.179 0.083
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant EG-VEGF and the recovery rates were calculated by comparing the measured value to the expected amount of EG-VEGF in samples.
Matrix Recovery range Average
serum(n=5) 90-103% 96%
EDTA plasma(n=5) 81-96% 88%
Heparin plasma(n=5) 78-94% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EG-VEGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 91-103% 87-98% 92-99%
EDTA plasma(n=5) 89-99% 83-96% 98-104% 88-101%
Heparin plasma(n=5) 85-102% 84-99% 97-101% 89-102%