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Rabbit PDGFA(Platelet Derived Growth Factor Subunit A) ELISA Kit
ELK2439
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48 T $412.00
96 T $588.00
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Overview

Product name: Rabbit PDGFA(Platelet Derived Growth Factor Subunit A) ELISA Kit
Reactivity: Rabbit
Alternative Names: PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide
Assay Type: Sandwich
Sensitivity: 10.8 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Bone metabolism;
Uniprot ID: P34007
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit PDGFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PDGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit PDGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit PDGFA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 1.994 1.905
1000.00 1.636 1.547
500.00 1.043 0.954
250.00 0.872 0.783
125.00 0.474 0.385
62.50 0.326 0.237
31.25 0.168 0.079
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PDGFA and the recovery rates were calculated by comparing the measured value to the expected amount of PDGFA in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 83-98% 90%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-98% 87-103% 89-97% 85-94%
EDTA plasma(n=5) 95-103% 79-97% 93-102% 87-103%
Heparin plasma(n=5) 93-106% 87-99% 85-93% 79-96%