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Human sIL-6R(SolubleInterleukin 6 Receptor) ELISA Kit
ELK2247
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48 T $320.00
96 T $458.00
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Overview

Product name: Human sIL-6R(SolubleInterleukin 6 Receptor) ELISA Kit
Reactivity: Human
Alternative Names: IL-6R;CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; Interleukin 6 Receptor
Assay Type: Sandwich
Sensitivity: 0.83 ng/mL
Standard: 120 ng/mL
Detection Range: 1.88-120 ng/mL
Sample Type: Serum, plasma, tissue homogenates, urine and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
120.00 2.204 2.135
60.00 1.700 1.631
30.00 1.121 1.052
15.00 0.875 0.806
7.50 0.543 0.474
3.75 0.387 0.318
1.88 0.156 0.087
0.00 0.069 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sIL-6R and the recovery rates were calculated by comparing the measured value to the expected amount of sIL-6R in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 88-102% 95%
Heparin plasma(n=5) 80-92% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sIL-6R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 87-96% 82-95% 92-105%
EDTA plasma(n=5) 90-99% 95-106% 87-101% 82-97%
Heparin plasma(n=5) 88-103% 83-94% 92-105% 89-97%