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Mouse PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
ELK2231
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Mouse PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
Reactivity: Mouse
Alternative Names: PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2; Prostaglandin Endoperoxide Synthase 2
Assay Type: Sandwich
Sensitivity: 35 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;Reproductive science;Pulmonology;Gastroenterology;Hormone metabolism;
Uniprot ID: Q05769
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.045 1.949
2500.00 1.577 1.481
1250.00 1.096 1.000
625.00 0.807 0.711
312.50 0.538 0.442
156.25 0.312 0.216
78.13 0.221 0.125
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PTGS2/COX-2 and the recovery rates were calculated by comparing the measured value to the expected amount of PTGS2/COX-2 in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 81-95% 88%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTGS2/COX-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-103% 82-93% 86-95% 81-96%
EDTA plasma(n=5) 92-104% 86-98% 80-93% 89-97%
Heparin plasma(n=5) 85-90% 83-96% 95-102% 88-95%