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Human Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit
ELK2100
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit
Reactivity: Human
Alternative Names: Amyloid beta 42(ABeta 42); amyloid beta peptide 1-42; Aβ1-42,amyloid βpeptide 1-42
Assay Type: Sandwich
Sensitivity: 5.31 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.161 2.078
500.00 1.641 1.558
250.00 1.234 1.151
125.00 0.969 0.886
62.50 0.543 0.460
31.25 0.351 0.268
15.63 0.291 0.208
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Ab1-42 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-42 in samples.
Matrix Recovery range Average
serum(n=5) 88-102% 95%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 80-92% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-42 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-96% 79-95% 95-102%
EDTA plasma(n=5) 85-92% 79-96% 87-103% 90-99%
Heparin plasma(n=5) 88-97% 96-105% 91-103% 89-97%