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Human PROC(Protein C) ELISA Kit
ELK2048
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48 T $320.00
96 T $458.00
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Overview

Product name: Human PROC(Protein C) ELISA Kit
Reactivity: Human
Alternative Names: PRO-C; VKDPC; PC; Vitamin K Dependent Protein C; Inactivator Of Coagulation Factors Va And VIIIa; Autoprothrombin IIA; Blood coagulation factor XIV; Activation peptide
Assay Type: Sandwich
Sensitivity: 24.8 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Plasma
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Hematology;Cardiovascular biology;
Uniprot ID: P04070
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PROC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PROC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PROC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PROC in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 2.121 2.038
2000.00 1.642 1.559
1000.00 1.243 1.160
500.00 0.839 0.756
250.00 0.521 0.438
125.00 0.395 0.312
62.50 0.156 0.073
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PROC and the recovery rates were calculated by comparing the measured value to the expected amount of PROC in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PROC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-101% 93-106% 85-95% 92-104%
EDTA plasma(n=5) 86-97% 83-96% 91-103% 96-105%
Heparin plasma(n=5) 87-98% 92-104% 82-96% 95-102%