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Human SCT(Secretin) ELISA Kit
ELK1753
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48 T $320.00
96 T $458.00
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Overview

Product name: Human SCT(Secretin) ELISA Kit
Reactivity: Human
Alternative Names: Secretin
Assay Type: Competitive Inhibition
Sensitivity: 7.14 pg/mL
Standard: 1500 pg/mL
Detection Range: 23.44-1500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Hepatology;Hormone metabolism;
Uniprot ID: P09683
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SCT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1500.00 0.227
750.00 0.386
375.00 0.611
187.50 0.833
93.75 1.156
46.88 1.511
23.44 1.789
0.00 2.211

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant SCT and the recovery rates were calculated by comparing the measured value to the expected amount of SCT in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 89-103% 96%
Heparin plasma(n=5) 78-96% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SCT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 87-96% 85-93% 82-90%
EDTA plasma(n=5) 85-101% 78-96% 79-98% 93-102%
Heparin plasma(n=5) 88-101% 90-98% 92-106% 85-97%