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Human GPIIb/IIIa(Glycoprotein IIb/IIIa) ELISA Kit
ELK1688
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48 T $320.00
96 T $458.00
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Overview

Product name: Human GPIIb/IIIa(Glycoprotein IIb/IIIa) ELISA Kit
Reactivity: Human
Alternative Names: GP-IIb/IIIa
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Metabolic pathway;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPIIb/IIIa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPIIb/IIIa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPIIb/IIIa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPIIb/IIIa in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 1.966 1.886
5.00 1.518 1.438
2.50 1.052 0.972
1.25 0.861 0.781
0.63 0.533 0.453
0.32 0.325 0.245
0.16 0.176 0.096
0.00 0.080 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant GPIIb/IIIa and the recovery rates were calculated by comparing the measured value to the expected amount of GPIIb/IIIa in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 84-102% 93%
Heparin plasma(n=5) 87-95% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPIIb/IIIa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-100% 93-108% 80-101% 87-96%
EDTA plasma(n=5) 86-97% 88-95% 92-101% 87-98%
Heparin plasma(n=5) 92-101% 82-98% 89-97% 85-93%