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Mouse TSP-1(Thrombospondin-1) ELISA Kit
ELK1687
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse TSP-1(Thrombospondin-1) ELISA Kit
Reactivity: Mouse
Alternative Names: TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1
Assay Type: Sandwich
Sensitivity: 0.266 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Hematology;
Uniprot ID: P35441
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
40.00 2.219 2.131
20.00 1.636 1.548
10.00 1.106 1.018
5.00 0.847 0.759
2.50 0.581 0.493
1.25 0.381 0.293
0.63 0.241 0.153
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TSP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of TSP-1 in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 90-103% 96%
Heparin plasma(n=5) 78-93% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-99% 93-107% 85-94% 87-101%
EDTA plasma(n=5) 79-96% 85-99% 79-93% 89-97%
Heparin plasma(n=5) 85-97% 87-96% 85-96% 85-95%