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Rat TSP-1(Thrombospondin-1) ELISA Kit
ELK1686
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat TSP-1(Thrombospondin-1) ELISA Kit
Reactivity: Rat
Alternative Names: TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1
Assay Type: Sandwich
Sensitivity: 3.6 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Plasma
Assay Length: 3.5h
Research Area: Infection immunity;Hematology;
Uniprot ID: Q71SA3
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.368 2.285
250.00 1.720 1.637
125.00 1.225 1.142
62.50 0.782 0.699
31.25 0.586 0.503
15.63 0.321 0.238
7.82 0.251 0.168
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TSP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of TSP-1 in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 92-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-98% 93-106% 89-97% 85-94%
EDTA plasma(n=5) 82-96% 85-98% 95-104% 82-96%
Heparin plasma(n=5) 85-96% 87-101% 89-97% 85-94%