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Rat CC16(Clara Cell Protein 16) ELISA Kit
ELK1642
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat CC16(Clara Cell Protein 16) ELISA Kit
Reactivity: Rat
Alternative Names: SCGB1A1; CC10; CCSP; UGB; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein
Assay Type: Sandwich
Sensitivity: 30 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Cardiovascular biology;Pulmonology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CC16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CC16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CC16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CC16 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.135 2.052
2500.00 1.562 1.479
1250.00 1.213 1.130
625.00 0.867 0.784
312.50 0.496 0.413
156.25 0.319 0.236
78.13 0.129 0.046
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CC16 and the recovery rates were calculated by comparing the measured value to the expected amount of CC16 in samples.
Matrix Recovery range Average
serum(n=5) 92-107% 99%
EDTA plasma(n=5) 88-102% 95%
Heparin plasma(n=5) 83-96% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CC16 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 87-98% 85-92% 79-96%
EDTA plasma(n=5) 86-96% 85-97% 89-101% 85-97%
Heparin plasma(n=5) 80-101% 87-96% 96-105% 95-103%