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Mouse NMB(Neuromedin B) ELISA Kit
ELK1630
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse NMB(Neuromedin B) ELISA Kit
Reactivity: Mouse
Alternative Names: NM-B
Assay Type: Competitive Inhibition
Sensitivity: 4.75 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Signal transduction;Endocrinology;Neuro science;Gastroenterology;Hormone metabolism;
Uniprot ID: Q9CR53
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NMB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NMB in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 0.219
500.00 0.427
250.00 0.715
125.00 0.959
62.50 1.275
31.25 1.579
15.63 1.889
0.00 2.265

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NMB and the recovery rates were calculated by comparing the measured value to the expected amount of NMB in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 84-97% 90%
Heparin plasma(n=5) 81-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NMB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 87-96% 82-93% 89-97%
EDTA plasma(n=5) 85-93% 98-105% 87-98% 95-102%
Heparin plasma(n=5) 85-94% 79-96% 92-105% 91-99%