Products

Rat GIP(Gastric Inhibitory Polypeptide) ELISA Kit
ELK1581
Size： Price：
48 T $320.00
96 T $458.00
Download ① Send email to us

Overview

Product name: Rat GIP(Gastric Inhibitory Polypeptide) ELISA Kit
Reactivity: Rat
Alternative Names: Incretin hormone;  Glucose Dependent Insulinotropic Peptide
Assay Type: Competitive Inhibition
Sensitivity: 27.1 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Gastroenterology;Hormone metabolism;
Uniprot ID: Q06145
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GIP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GIP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 0.185
2500.00 0.389
1250.00 0.621
625.00 0.833
312.50 1.119
156.25 1.565
78.13 1.813
0.00 2.101

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant GIP and the recovery rates were calculated by comparing the measured value to the expected amount of GIP in samples.
Matrix Recovery range Average
serum(n=5) 88-99% 93%
EDTA plasma(n=5) 86-98% 92%
Heparin plasma(n=5) 82-98% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-104% 97-106% 82-91% 85-96%
EDTA plasma(n=5) 85-93% 87-96% 97-105% 95-102%
Heparin plasma(n=5) 78-92% 79-93% 94-106% 83-97%