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Rat Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit
ELK1494
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit
Reactivity: Rat
Alternative Names: Amyloid Beta Peptide 1-40
Assay Type: Sandwich
Sensitivity: 4.84 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Ab1-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Ab1-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Ab1-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Ab1-40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 1.933 1.839
500.00 1.525 1.431
250.00 1.028 0.934
125.00 0.899 0.805
62.50 0.601 0.507
31.25 0.345 0.251
15.63 0.196 0.102
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Ab1-40 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-40 in samples.
Matrix Recovery range Average
serum(n=5) 93-107% 100%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 85-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 90-99% 82-101% 93-105%
EDTA plasma(n=5) 82-95% 88-102% 84-97% 89-107%
Heparin plasma(n=5) 95-105% 82-95% 81-97% 93-105%