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Human Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit
ELK1492
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit
Reactivity: Human
Alternative Names: Amyloid Beta Peptide 1-40
Assay Type: Sandwich
Sensitivity: 4.58 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ab1-40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.082 1.987
500.00 1.621 1.526
250.00 0.997 0.902
125.00 0.765 0.670
62.50 0.503 0.408
31.25 0.351 0.256
15.63 0.221 0.126
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Ab1-40 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-40 in samples.
Matrix Recovery range Average
serum(n=5) 80-94% 87%
EDTA plasma(n=5) 89-101% 95%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 86-102% 94-106% 85-97%
EDTA plasma(n=5) 85-97% 93-106% 83-95% 88-103%
Heparin plasma(n=5) 95-103% 88-96% 81-95% 91-102%