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Human GC(Glucagon) ELISA Kit
ELK1081
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48 T $265.00
96 T $376.00
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Overview

Product name: Human GC(Glucagon) ELISA Kit
Reactivity: Human
Alternative Names: GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone
Assay Type: Competitive Inhibition
Sensitivity: 7.54 pg/mL
Standard: 1500 pg/mL
Detection Range: 23.44-1500 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;
Uniprot ID: P01275
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GC in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1500.00 0.191
750.00 0.275
375.00 0.446
187.50 0.598
93.75 0.763
46.88 1.201
23.44 1.624
0.00 2.028

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant GC and the recovery rates were calculated by comparing the measured value to the expected amount of GC in samples.
Matrix Recovery range Average
serum(n=5) 93-105% 99%
EDTA plasma(n=5) 84-93% 89%
Heparin plasma(n=5) 81-103% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 85-97% 87-96% 85-96%
EDTA plasma(n=5) 95-103% 89-101% 87-98% 85-92%
Heparin plasma(n=5) 85-97% 86-102% 87-99% 99-105%