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Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit
ELK0960
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit
Reactivity: Rat
Alternative Names: Bsk; BSK; CG5680; D-JNK; D-junk; DBSK/JNK; dJnk; dJNK; DJNK; DJNK/bsk; Dmel\CG5680; DmJNK; dNJNK; jnk; Jnk; JNK; jnk (bsk); JNK/SAPK; Junk; p-JNK; P-JNK; pJNK; SAPKa
Assay Type: Sandwich
Sensitivity: 0.146 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-JNK in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.201 2.114
10.00 1.638 1.551
5.00 1.182 1.095
2.50 0.921 0.834
1.25 0.532 0.445
0.63 0.369 0.282
0.32 0.216 0.129
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant p-JNK and the recovery rates were calculated by comparing the measured value to the expected amount of p-JNK in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 92-107% 99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p-JNK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-105% 88-102% 93-101% 83-94%
EDTA plasma(n=5) 92-105% 85-102% 98-105% 88-97%
Heparin plasma(n=5) 85-98% 93-102% 89-97% 85-94%