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Rat Lp-a (Lipoprotein A) ELISA Kit
ELK0948
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat Lp-a (Lipoprotein A) ELISA Kit
Reactivity: Rat
Alternative Names: LP; AK38; APOA
Assay Type: Sandwich
Sensitivity: 0.72 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Metabolic pathway;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Lp-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Lp-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Lp-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Lp-a in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.131 2.035
50.00 1.668 1.572
25.00 1.088 0.992
12.50 0.813 0.717
6.25 0.559 0.463
3.13 0.357 0.261
1.57 0.236 0.140
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Lp-a and the recovery rates were calculated by comparing the measured value to the expected amount of Lp-a in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 85-99% 92%
Heparin plasma(n=5) 82-98% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lp-a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 91-102% 79-91% 89-103% 82-95%
EDTA plasma(n=5) 79-92% 87-103% 79-96% 92-105%
Heparin plasma(n=5) 85-92% 96-103% 87-98% 95-106%