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Human B2-glycoprotein 1 (β2-GP1) antibody lgA ELISA Kit
ELK0937
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48 T $320.00
96 T $458.00
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Overview

Product name: Human B2-glycoprotein 1 (β2-GP1) antibody lgA ELISA Kit
Reactivity: Human
Alternative Names: APOH; BG; B2G1; B2GP1
Assay Type: Sandwich
Sensitivity: 26.3 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human β2-GP1 lgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human β2-GP1 lgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human β2-GP1 lgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human β2-GP1 lgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 1.925 1.842
2000.00 1.668 1.585
1000.00 1.226 1.143
500.00 0.796 0.713
250.00 0.559 0.476
125.00 0.318 0.235
62.50 0.258 0.175
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant β2-GP1 lgA and the recovery rates were calculated by comparing the measured value to the expected amount of β2-GP1 lgA in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β2-GP1 lgA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 92-105% 83-92% 85-92%
EDTA plasma(n=5) 82-97% 88-105% 83-96% 82-92%
Heparin plasma(n=5) 92-101% 80-95% 87-96% 89-99%