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Human B2-glycoprotein 1 (β2-GP1) antibody lgG ELISA Kit
ELK0936
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48 T $320.00
96 T $458.00
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Overview

Product name: Human B2-glycoprotein 1 (β2-GP1) antibody lgG ELISA Kit
Reactivity: Human
Alternative Names: APOH; BG; B2G1; B2GP1
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human β2-GP1 lgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human β2-GP1 lgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human β2-GP1 lgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human β2-GP1 lgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 1.994 1.909
5.00 1.612 1.527
2.50 1.175 1.090
1.25 0.864 0.779
0.63 0.539 0.454
0.32 0.319 0.234
0.16 0.236 0.151
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant β2-GP1 lgG and the recovery rates were calculated by comparing the measured value to the expected amount of β2-GP1 lgG in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 84-96% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β2-GP1 lgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 85-94% 87-101% 95-102%
EDTA plasma(n=5) 87-103% 89-98% 81-95% 98-105%
Heparin plasma(n=5) 83-99% 80-96% 91-101% 89-100%